Figure 2

Effects of cimetidine on NCAM and NF-κB gene expression. A, the relative quantities of both NCAM and NF-κB mRNA were suddenly decreased by treatment with 10^-8 M cimetidine for 24 h, and then kept decreasing slightly by treatment with the higher doses of it. B, the relative quantity of NCAM mRNA was rapidly decreased in the first 15 min, and then continued to decrease in a time-dependent manner up to 24 h. However, the relative quantity of NF-κB mRNA showed almost no variation up to 8 h, and after that decreased at 24 h. C, initially, the relative quantity of NF-κB mRNA was constitutively induced in HSG cells and was gradually increased upon stimulation with TNF-α, an effect that reached a peak at 60 min and then decreased up to 24 h in a time-dependent fashion. With an increasing quantity of NF-κB mRNA, the relative quantity of NCAM mRNA was gradually increased upon stimulation with TNF-α up to 24 h in a time-dependent manner. D, NF-κB siRNA greatly reduced NCAM mRNA expression in HSG cells, in comparison with transfection using NF-κB scrambled siRNA. Each column and bar represents the mean ± S.E.M. of three independent experiments.