Figure 5

PKCε is necessary for migration of SK-N-BE(2)C neuroblastoma cells. (A) Cells were transfected with three different siRNA oligonucleotides against PKCε and the expression levels of PKCε were detected with Western blot. (B and C) Cells seeded in 35-mm cells culture wells were transfected with the PKCε siRNA oligonucleotides, one oligonucleotide against PKCδ or with an equal amount of water (Ctrl). Cells were incubated for 18 h after the last transfection and thereafter the confluent cell monolayer was scraped with a pipette tip. Medium with serum (B) or 16 nM TPA (C) was added to the wells. Data are presented as relative values of the migration of control cells after 24 h and are mean ± SEM of three independent experiments. (D) Data are presented as relative values where TPA at the 12 h time point is 100% and are mean ± SEM of three independent experiments. * denotes significant difference for all PKCε siRNAs compared to control conditions (B and C) and significant difference compared to absence of TPA (D).