Figure 4

PKCε mediates migration of SK-N-BE(2)C neuroblastoma cells. (A) Cells were transfected with 50 nM siRNA oligonucleotides against PKCα, PKCδ and PKCε and the expression levels of the proteins were detected with Western blot. (B and C) SK-N-BE(2)C cells were treated with indicated siRNA and 18 hours after the last transfection 15,000 cells were seeded in the upper wells in a migration chamber. Cells were allowed to migrate towards serum-free medium (B) or 16 nM TPA (C) for 6 h. Data (mean ± SEM, n = 9 [B] or n = 11 [C]) represent the number of migrated cells expressed as the percent of migration obtained with cells that had been treated with no siRNA oligonucleotide. * denotes significant difference compared to control conditions.