Figure 3

Activation of PKC reduces the wound closure time of SK-N-BE(2)C neuroblastoma cells. A confluent SK-N-BE(2)C cell monolayer was scraped with a pipette tip. (A-E) Cells were incubated in medium supplemented with serum (Ctrl) (A) or medium supplemented with serum and 16 nM TPA (B), or with the additional supplementation of PKC inhibitors GF109203X (2 μM) (C), Gö6976 (2 μM) (D) or LY333531 (200 nM) (E). Cells were incubated at 37°C for 48 hours and images of the scratches were captured. (F and G) Quantification of migration in the presence (F) or absence (G) of TPA. Data are presented as relative values where the 24 h timepoint for cells incubated in the absence of TPA is 100% and are mean ± SEM of four (F) or five (G) independent experiments. * denotes significant difference compared to absence of inhibitors.