Figure 1
3 H-thymidine incorporation of melanoma cells prior to and following treatment with 200 Gy gamma-irradiation. Human melanoma deposits were resected surgically and the tumors rendered into single cell suspensions by mechanical and enzymatic dissociation. The cell suspensions were then prepared into a tumor cell vaccine and split into samples receiving or not receiving 200 Gy gamma-irradiation. The non-irradiated and irradiated cells were then cultured in vitro for five days prior to the addition of 3H-thymidine for 18 to 24 hr. (A) Proliferation in the absence of gamma-irradiation. Samples ordered from lowest to highest proliferation. (B) Proliferation following treatment with 200 Gy gamma-irradiation. The data are presented as percent maximal 3H-thymidine incorporation in comparison to the non-irradiated control. Samples are ordered as in (A). The dashed horizontal bar indicates 5% maximal 3H-thymidine incorporation.