Table 1 Antibodies and scoring system used for analysis
Antibody* | Dilution | Clone | Method of scoring |
|---|---|---|---|
ER-α | 1:300 | 1D5 | > 10% positive cells – positive, < 10%- negative |
PR | 1:400 | PgR636 | > 10% positive cells – positive, < 10%- negative |
HER-2/neu | 1:200 | ** | Scored as +1, +2 and +3, +3- positive, +1/+2 -negative |
COX-2 | 1:100 | CX229 | Strong positive, weak positive, negative |
PPARγ | 1:50 | E8 | Nuclear, cytoplasmic, nuclear+cytoplasmic, negative |
P53 | 1:200 | DO-7 | > 10% positive cells – positive, < 10% -negative |
P21 | 1:50 | SX118 | > 10% positive cells – positive, < 10% -negative |
Cyclin D1 | 1:300 | DCS-6 | > 10% positive cells – positive, < 10% -negative |