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Table 4 Reference genes ranked in order of increasing expression stability in paired liver tissues*.

From: Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

paired tissues A (n = 22)

Paired tissues B (n = 32)

geNorm

M value

NormFinder

Stability value

geNorm

M value

NormFinder

Stability value

B2M

0.799

B2M

0.149

B2M

0.826

UBC

0.207

HPRT1

0.658

HPRT1

0.105

HPRT1

0.816

SDHA

0.203

UBC

0.654

UBC

0.101

UBC

0.771

HPRT1

0.164

SDHA

0.598

GAPDH

0.068

SDHA

0.711

B2M

0.159

GAPDH

0.566

SDHA

0.052

GAPDH

0.692

GAPDH

0.145

HMBS

0.514

HMBS

0.021

HMBS

0.629

HMBS

0.084

HMBS-GAPDH

0.417

HMBS-SDHA

0.033

HMBS-SDHA

0.489

HMBS-B2M

0.079

  1. Paired tissues A: paired tumoral and non-tumoral tissues left after the exclusion of RNA samples with relatively high amount of inhibitors; Paired tissues B: all paired tissues. *Increases from top to bottom.
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BMC Cancer

ISSN: 1471-2407

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