Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

Table 3 Pearson's correlation analysis between the existence of qRT-PCR inhibitors and OD 260/230.

	median	75th	Mean	STD	Normality test	Pearson's correlation coefficient
ΔCt*	0,49	0.675	0.47	0.26	P ≥ 0.20	r = -0.2420 (r² = 0.0586), p = 0.175
OD 260/230	1.49	1.965	1.49	0.48	P ≥ 0.15

Both of the variables were determined to be approximately normally distributed prior to the calculation of Pearson's correlation coefficient. * Amplification of alien RNA in 20 ng RNA samples compared with alien RNA alone; OD260/230: the ratio of optical density (OD) at wavelength of 260 nm to that at 230 nm. 75th: 75th percentile.

ISSN: 1471-2407