Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

Table 2 Details of primers and amplicons for the 6 evaluated genes.

Gene	Primer	Sequence (5' → 3')	Genomic Position	Amplicon Length	correlation coefficients (R²)	qRT-PCR Efficiency (%)
B2M	Forward	CTCCGTGGCCTTAGCTGTG	1st Exon	69 bp	0.998	90.1
	Reverse	TTTGGAGTACGCTGGATAGCCT	1st/2nd exon
GAPDH	Forward	TGCACCACCAACTGCTTAGC	7th Exon	87 bp	0.997	92
	Reverse	GGCATGGACTGTGGTCATGAG	7th/8th exon
HMBS	Forward	TGCAACGGCGGAAGAAAA	1st/2nd Exon	113 bp	0.998	91.0
	Reverse	ACGAGGCTTTCAATGTTGCC	3rd exon
HPRT1	Forward	TGACACTGGCAAAACAATGCA	6th Exon	94 bp	0.997	94.9
	Reverse	GGTCCTTTTCACCAGCAAGCT	6th/7th exon
SDHA	Forward	TGGGAACAAGAGGGCATCTG	2nd Exon	86 bp	0.996	87.8
	Reverse	CCACCACTGCATCAAATTCATG	3rd exon
UBC	Forward	CGGTGAACGCCGATGATTAT	1st Exon	124 bp	0.994	88.4
	Reverse	ATCTGCATTGTCAAGTGACGA	1st/2nd exon

ISSN: 1471-2407