Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

Table 1 Putative reference genes evaluated.

Gene	GenBank accession number	Name	Function	Genomic localization	Gene Aliases	IMAGE
B2M	NM_004048	Beta-2-microglobulin	Beta-chain of major histocompatibility complex class I molecules	15q21-q22		51940
GAPDH	NM_002046	Glyceraldehyde-3-phosphate dehydrogenase	Oxidoreductase in glycolysis and gluconeogenesis	12p13	G3PD	510510
HMBS	NM_000190.3	Hydroxymethyl-bilane synthase	Heme synthesis, porphyrin metabolism	11q23		245564
HPRT1	NM_000194.1	Hypoxanthine phosphoribosyl-transferase 1	Purine synthesis in salvage pathway	Xq26	HGPRT	345845
SDHA	NM_004168.2	Succinate dehydrogenase complex, subunit A	Electron transporter in the TCA cycle and respiratory chain	5p15	FP; SDH2; SDHF	375812
UBC	NM_021009.3	Ubiquitin C	Protein degradation	12q24		510582

ISSN: 1471-2407