Figure 2

QRT-PCR inhibitor detection in standards and several RNA samples. ΔCt for amplification of alien RNA in standards spiked with 20 ng E. coli total RNA and 20 ng RNA from several RNA samples were compared with that of alien RNA alone. The tissue samples contained relatively high and low abundance of qRT-PCR inhibitor existence as determined by the qRT-PCR detection among all samples. Each sample was spiked with 10⁵ copies of alien RNA transcripts.