Figure 5From: Activation of MEK1 or MEK2 isoform is sufficient to fully transform intestinal epithelial cells and induce the formation of metastatic tumors Constitutive activation of MEK1 or MEK2 confers intestinal epithelial cells resistance to anoikis. (A) IEC-6 cell populations infected with vector alone or activated MEK1 or MEK2 were cultured in suspension on poly-HEMA-coated plates for the indicated times. Apoptosis was evaluated by TUNEL assay. (B and C) Expression of Bcl-2 anti-apoptotic (B) and pro-apoptotic (C) family proteins was analyzed by immunoblotting in cell extracts from IEC-6 cell populations described in A. Total actin was used as loading control.Back to article page