Figure 4
From: Methylation of WTH3, a possible drug resistant gene, inhibits p53 regulated expression
SQRT-PCR results for endogenous WTH3 gene expression in MCF7/AdrR and Hela cells. A. Electrophoresis gel display of the endogenous WTH3 gene expression in MCF7/AdrR cells that were transfected with pcDNA3.1 or p53, and transfected with pcDNA3.1 or p53 and treated with 5-aza for 24 or 72 hr. GAPDH served as positive control. B. Results of quantitative analysis for MDR1 expression in the cells presented in A. GAPDH served as quantitative reference.