Figure 3

Expression of tumour markers in KGN at different passages. Western blot analysis of KGN cells at P7 and P51 was conducted to examine the expression level of p53, osteopontin, BAX and BAG-1. Ten μg (for BAX and BAG-1) or 50 μg (for p53 and osteopontin) of whole cell extract were separated on a 12.5% reducing gel. The lower panel represents the mean intensity of each band normalised by dividing with the intensity of β-actin.