Figure 2

Proliferation of KGN cells at different passages. The early (P6) and advanced (P58) passage KGN cells were cultured in 10% delipidated FBS, 0.1 IU/ml LH, or 1 IU/ml FSH, seeded at 1 × 10⁴ cells/well in 96-well plates and subjected to the proliferation assay. The experiments were triplicated and the data are shown as the mean absorbance with SEM (at OD 450 nm). Statistical analysis was conducted using Student's t-test.