Figure 5

BOP1 and SMAD4 immunohistochemical staining. A. Example of BOP1 immunohistochemical staining in two carcinomas; the left picture shows a weak expression (IHC score 1), the right picture, a very strong expression (score 3). B. Correlation plot of BOP1 mRNA expression (x-axis) and BOP1 protein expression (y-axis) (left) and BOP1 immunohistochemical staining (y-axis) related to 8q gain(x-axis) (right). C. Example of SMAD4 immunohistochemical staining in two carcinomas; the left picture shows loss of SMAD4 expression (score 1), the right picture positive expression (score 3). D. Correlation plot of SMAD4 gene expression (x-axis) and SMAD 4 protein expression (y-axis) (left) and correlation plot of SMAD2 gene expression (x-axis) and SMAD 4 protein expression (right) (y-axis).