Figure 6
From: STAT3 can be activated through paracrine signaling in breast epithelial cells
Inhibition of IL-6 or gp130 is sufficient to block increased levels of p-STAT3 (Y705) in MDA-MB-453. A. MDA-MB-231 conditioned media was subjected to 4 μg/ml IL-6, gp130, or GAPDH antibody neutralization. This neutralized conditioned media was then used to treat MDA-MB-453 cells for 0.5 hours. B. BCF conditioned media was subjected to 4 μg/ml IL-6, gp130, or GAPDH antibody neutralization. This neutralized conditioned media was then used to treat MDA-MB-453 cells for 0.5 hours. 453: MDA-MB-453; 231: MDA-MB-231; BCF: breast cancer associated fibroblasts; No Tx: no treatment; CM: conditioned media.