Figure 4

Six days RA treatment lowers DCX and LIS1 protein levels while NF-68 content increases. WB representative images and results of the densitometric analysis (A-D). After 6 days RA treatment the protein level of DCX (A) decreases by 87.4% and that of LIS1 (B) by 38.4%; NF-68 protein amount increases by 86.3% (C). Data related to VIM (D) show high variability and do not indicate any change in protein level after RA treatment. C, controls; RA, samples treated with 10 μM RA for 6 days. Bars represent mean ± standard error, N = 8 and data are expressed as a percentage of the control (100%); single star = p < 0.05; double star = p < 0.01.