Mutation of RAS , PTPN11 and RUNX1 genes in CMML. Examples of mutations in candidate genes. From top to bottom, sequence of the mutated KRAS, PTPN11, SOS1 and RUNX1 alleles, demonstrating base change in the forward sequence at the position indicated by an arrow. The corresponding sequence is shown above. Primers and conditions used are described in additional file 2.