Figure 2

Schematic depiction of three shRNA: Three pairs of oligonucleotides were designed and were synthesized by BIO BASIC Inc (Shang Hai, China). shRNA1 corresponds to positions 256–274 of the CDC2 open reading frame, shRNA3 to positions 789–807 and shRNA4 to positions 909–927. the following were the sequence of three pairs of oligonucleotides: Oligo 1:forward 5'-GAT CCC CGG GGT TCC TAG TAC TGC AAT TCA AGA GAT TGC AGT ACT AGG AAC CCC TTT TTA-3'; reverse5'-AGC TTA AAA AGG GGT TCC TAG TAC TGC AAT CTC TTG AAT TGC AGT ACT AGG AAC CCC GGG-3'; Oligo 3:forward 5'-GAT CCC CGG GCA CTC CCA ATA ATG AAT TCA AGA GAT TCA TTA TTG GGA GTG CCC TTT TTA-3', reverse 5'-AGC TTA AAA AGG GCA CTC CCA ATA ATG AAT CTC TTG AAT TCA TTA TTG GGA GTG CCC GGG-3'; Oligo 4: forward 5'-GAT CCC CCT TGG ATT TGC TCT CGA AAT TCA AGA GAT TTC GAG AGC AAA TCC AAG TTT TTA-3', reverse5'-AGC TTA AAA ACT TGG ATT TGC TCT CGA AAT CTC TTG AAT TTC GAG AGC AAA TCC AAG GGG-3'.