Figure 6
Cellular and subcellular compartmentalization of caveolin 1 (Cav-1) and β-catenin expression. A. Cav-1 expression. a-d – Immunohistochemical analysis of Cav-1 expression in mammary gland and tumors. Cav-1 is stained in epithelial cells of the carcinoma tumors vs. the fibrovascular core of the papillary adenocarcinoma tumors. Arrows mark Cav-1 expression in the parenchymatic cells. Asterisk marks Cav-1 expression in fibroblasts. Bar = 20 μm. Inset: 3× magnification. e-h – Immunofluorescent green staining of Cav-1 supports its differential localization in the parenchymatic cells of the carcinoma vs. the fibrovascular core of the papillary adenocarcinoma. Bar = 10 μm. i-l – Immunofluorescence staining of Cav-1 (red) and SMA (green) indicates differential expression in the tumor cells. Bar = 20 μm. Inset: 3× magnification. B. Immunofluorescence localization of β-catenin in mammary gland and tumor cells. Arrows mark nuclear-localized β-catenin in the carcinoma tumors. Arrowhead indicates lack of membranal expression of β-catenin. Bar = 10 μm.