Figure 4
From: Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres
Quantitative real-time PCR shows that restoration of miR-34 by miR-34 mimic transfection downregulates target gene expression. 24 hours after miR-34 mimic transfection of Kato III cells (100 pmol per well in 6-well plates), potential target gene mRNA levels were measured by qRT-PCR with SYBR Green PCR system (TaqMan). The comparative threshold cycle CT method was used to calculate relative gene expression levels compared with actin, then normalized with the value from NC mimic as 1.