Figure 5
From: Conjugated linoleic acid induces apoptosis through estrogen receptor alpha in human breast tissue
CLA decreases E 2 stimulated Bcl-2 protein expression in ERα(+) breast cells. (A) Immunohistochemistry of Bcl-2 staining (brown to dark) in ERα(+) normal human breast tissue for 3 days of treatment. (B) Western blot analysis of basal ERα protein expression in MCF-7 and ERα(+) NEC, and the effects of CLA and E2 on Bcl-2 protein expression in ERα(+) NEC for 3 days of treatment. β-actin was used as loading control. (C) Western blot analysis of basal ERα protein expression in MCF-7 and ERα positive human breast cancer epithelial cells (ERα(+) CAEC). And Bcl-2 protein expression in MCF-7 and ERα(+) CAEC was detected by western blot analysis after 3 days of treatment. CLA exerts combinative effects with Tam in decreasing E2 stimulated Bcl-2 protein expression in ERα(+) CAEC. CLA stands for 40 μM t10, c12-CLA; E2 stands for 10 nM 17β-estradiol.