Figure 2

Caspase 3 cleavage in hepatocytes of different genotypes. Cleaved-caspase 3 was labelled using red immunofluorescence and the proportion of positive cells quantified by counting 3 × 20 fields of view per genotype. B: The figure shows representative photos of (a, b) untreated hepatocytes 72 hours after plating (here Rb-/-); (c, d) hepatocytes in culture for 72 hours treated with TGFβ for 48 hours (p 53-/- & control); (e) untreated hepatocytes 96 hours after plating (p21 ^cip1-/-); (f, g, h) hepatocytes in culture for 96 hours treated with TGFβ for 72 hours (p53 p21 ^cip1 -/-, control &p21 ^cip1-/-).