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Figure 2 | BMC Cancer

Figure 2

From: Suppression subtractive hybridization profiles of radial growth phase and metastatic melanoma cell lines reveal novel potential targets

Figure 2

Subtracted cDNA profiles of the RGP and metastatic (Met) cells. PCR-1 represents the PCR products generated using a single primer directed towards both adaptors, after 27 amplification cycles from two duplicate samples of subtracted (S1 and S2) or non-subtracted (NS) cDNA of the RGP (WM1552C) and the metastatic (a pool of WM9, WM852, 1205Lu and WM1617) cell lines. PCR-2 represents the PCR product generated after 10 amplification cycles by nested-PCR using a specific primer for each adaptor. Note the difference between the subtracted and non-subtracted profiles.

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BMC Cancer

ISSN: 1471-2407

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