Figure 5
From: Rapamycin delays growth of Wnt-1 tumors in spite of suppression of host immunity
Effect of Rapamycin on mTOR signalling in Wnt-1 cells. A. Cells from Wnt-1 cultured for 3–4 days were trypsinyzed, seeded into flasks in complete medium and incubated overnight. Rapamycin (1 μM) was added for additional 24 hours. Western blots of cellular extracts were stained with specific antibodies to phosphorelated forms of TOR pathway messengers. B. Western blots of cell extracts from Wnt-1 cell lines, W1308 and W1204.