Figure 5

Comparison of lysosomal and endosomal acidification. Comparison of lysosomal and endosomal acidification in A2780 and A2780cis cells using ratios of emission intensities at 520 nm at the two excitation wavelengths (485 nm and 460 nm) after FITC-dextran labelling of endosomes and lysosomes, respectively. The results are expressed as means ± SE of emission intensity ratios in 24 wells (endosomes) and 48 wells (lysosomes) from two independent experiments. The significance of differences was analysed using Student's t test.