Figure 3
Immunohistochemical detection of hPXR and RXR receptors. Immunoreaction to hPXR1.2 (A, B, C and D). In ductal hyperplasia (A) (×250) and ductal carcinoma in situ (B) (×250), the reaction was observed in the cytoplasm. In infiltrative ductal carcinoma (C) (×250) and infiltrative lobular carcinoma (D) (×500), the hPXR1.2 immunoexpression was also observed in the nucleus of neoplastic cells. Immunoreaction to hPXR1 (E, F, G and H). Lobular hyperplasia (E) (×200) showing no immunoreaction to hPXR1 antibody. Micrograph of lobular carcinoma in situ (F) (×200), infiltrative ductal carcinoma (G) (×200) and infiltrative lobular carcinoma (H) (×200) with cytoplasmic immunolocation of hPXR1 isoform. Immunoreaction to RXR-α (I, J, K and L). Cytoplasmic immunolabelling of RXR-α in samples of ductal hyperplasia (I) (×200) and ductal carcinoma in situ (J) (×300). Neoplastic cells from infiltrative ductal carcinoma (K) (×300) and infiltrative lobular carcinoma (L) (×500) presenting a nuclear immunoexpression to RXR-α.