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Figure 2 | BMC Cancer

Figure 2

From: The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications

Figure 2

2D-PAGE distribution pattern of a panel of recombinant ( E. coli ) S100A4 proteins. One μg recombinant protein was separated by 2D-PAGE and visualized by western blots staining with a polyclonal antibody recognizing both mouse and human S100A4. A: Human S100A4 produced in-house using vector pGEX 3X with removal of GST-tag (previously shown in fig. 1E). B: Human his-tagged S100A4 produced in the lab of Prof. E. Lukanidin (Danish Cancer Society). C: Human S100A4 produced by Jena Biosciences. D: Mouse his-tagged S100A4 produced in-house using vector pQE30.

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BMC Cancer

ISSN: 1471-2407

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