Figure 1

Western blot of S100A4 separated by 2D-PAGE. S100A4 was immunoprecipitated from the complex samples using MAb 20.1 and blotted onto PVDF filters. The western blots are visualized by staining with MAb 22.3 against S100A4 and aligned according to the antibody light chain (not shown) detached during the immunoprecipitation procedure or added directly into the recombinant samples. A. Comparison of S100A4 in nuclear and cytoplasmic fractions isolated from in vitro cultured colorectal cancer cell line SW620 and extracellular S100A4 from conditioned growth medium by confluently growing SW620 cells. B. Comparison of S100A4 isolated from whole cell lysate of two in vitro cultured colorectal cancer cell lines with different TP53 status, SW620 (TP53 mut) and HCT116 (TP53 wt), and the OHS osteosarcoma cell line. C. Analysis of S100A4 isolated from biopsies collected from colorectal cancer patients diagnosed with different p53 status and Duke's stage. Two representative samples out of eight analyzed are shown. D. Endogenous S100A4 isolated from red blood cells (RBC) and mononuclear cells (MNC). E. Recombinant human S100A4 produced in-house using vector pGEX 3X (with removal of GST-tag).