Figure 4
Immunohistochemical staining for Bcl-2 (A-F) and Bax (G) expression in treatment groups and control group. Sections showed strong Bcl-2 cytoplasmic staining of tumor cells in (A) control group and (B) S-ODN group, weak Bcl-2 cytoplasmic staining in (C) As group, (D) ATRA group and (E) S/ATRA group, extremely weak Bcl-2 staining in (F) As/ATRA treated group. (G) Similar moderate Bax cytoplasmic staining of tumor cells in all treatment groups. Representative section of As/ATRA group is shown. Scale bar = 50 μm. Computer-assisted quantitation of immunohistochemical staining was performed. (H) The average IODs of Bcl-2 and Bax were shown as the mean ± SE (n = 10). The average IODs in As-ODN and ATRA treated groups were significantly decreased as compared to S-ODN group and control group. * P < 0.01, one-way ANOVA. Significant interaction was observed between As-ODN-hTR and ATRA treatment. * * F = 35.836, P < 0.01, two-way ANOVA. There was no significant difference of Bax expression in all treatment groups.