Figure 2

Cell apoptosis in treatment groups and control group. (A-F) Apoptotic cells were revealed by TUNEL-histochemistry assay. Representative microphotographs of Tunel staining of (A) control group, (B) S group, (C) As group, (D) ATRA group, (E)S/ATRA group and (F) As/ATRA group. Arrows indicate positive cells. Scale bar = 50 μm. The average Integrated Optical Density (IOD) of each group was performed by a computer-assisted quantitation. (G) IOD data is shown as the mean ± SE (n = 10). The average IODs in As-ODN and ATRA treated groups were significantly increased as compared to S-ODN group and control group. * P < 0.01, one-way ANOVA. Significant interaction was observed between As-ODN and ATRA treatment. * * F = 45.918, P < 0.01, two-way ANOVA. (H) Electron microscopy showed typical feature of apoptotic cell in As-ODN and ATRA treatment groups. Whereas, no ultrastructural changes were observed in S-ODN group and control group (data not shown).