Figure 7

Interactions of exogenously expressed SOCS3 mutants with PYK2 in A549 cells. A549 cells were transfected with either vector or SOCS3 mutants with distinct functional domains deleted, and pretreated with β-lactacystin 4 h before transfection until the cell lysates were subjected to anti-myc tag immunoprecipitations and analysed by western blot using the anti-PYK2 antibody. The co-immunoprecipitation product was clearly observed in the SOCS-box mutant transfected cells. The results are representative of three individual experiments.