Figure 4

Difference plots and sequence traces for KRAS exon 2 mutations. In the difference plot for KRAS exon 2, the melting curves from the four samples (TX23 in purple, TX41 in green, TX55 in orange and TX64 in brown) and a positive mutation control (RPMI8226 in red) were compared to a wild-type sample. The wild-type control sample is shown as a horizontal line in blue. Four patients and the positive control samples had different melting profiles compared to the wild-type. Sequencing of the patients revealed four different amino acid changes at codon 12; p.G12D in TX23, p.G12F in TX41, p.G12V in TX55 and p.G12C in TX64.