Figure 1

Difference plots showing mutation detection with various amounts of starting template. In difference plots, the melting profile of a wild type control is chosen as a horizontal base line and the relative differences in the melting of all the other samples are plotted relative to this baseline. Panel A: A difference plot of EGFR exon 19 using different amounts of template from one sample. Different amounts of template, ranging from 1 ng to 100 ng, were assessed by the EGFR exon 19 assay using a sample with a p.E746_A750 deletion mutation. A near identical heteroduplex type melting pattern is observed from each amount of template tested. The wild-type control sample is shown as a horizontal line (in blue). Panel B: A difference plot of EGFR exon 19 using different samples with different amounts of template. Each sample contained the same p.E746_A750 in-frame deletion mutation but the samples were at different DNA concentrations and from different individuals. A wild-type control sample is shown as a horizontal line (in blue). All samples show different melting patterns compared to the wild-type.