Expression of endocan and endocanΔ2 spliced products of esm1 gene. A: Scheme of the cloned human esm1 gene. (GenBank Accession Numbers AJ401091 and AJ401092). B: RT-PCR using primers hybridizing with sequences in exon 1 and 3 of esm1. Lane 1: endocan-HEK 293 cell line, lane 2: endocanΔ2-HEK 293 cell line, lane 3: HUVEC, lane 4: monkey lung, lane 5: rat lung, lane 6: mouse lung. The numbers to the left are molecular sizes in bp. Black arrows represent the endocan cDNA and white arrow represents endocanΔ2 cDNA.