A novel diffuse gastric cancer susceptibility variant in E-cadherin (CDH1) intron 2: A case control study in an Italian population

Table 1 PCR primers and conditions

	Forward primer	Reverse primer	T_a*	Mg^++†	DMSO^‡
C1F1	ccgccttaaagaaactcttg	accggtggcaaatactag	65°C	1.5	-
C1F2	tagaagggttgaacctgttc	tcttagtccacgagaagaag	65°C	1.5	-
C1F3	taggagagcttgtaacaagc	cactcggttctaccgaag	65°C	1.5	-
C2F1	tgtattagccacagagaag	ctaaaactagaccacgaag	65°C	1.5	-
C2F2	gtcacaaaacagcttg	ccttccttgagcaaggc	65°C	1.5	-
C3F1	ttgcctaaggccccctttttgttc	gaatctgcgaagtctacatc	65°C	1.5	-
C3F2	acactagccacacatgggactcaag	tgctggtgtggattcaaatgtg	65°C	1.5	-
C4F1	acctccgcctcctgggttcaagc	ttcctcccgcttagtg	60°C	1.5	-
C4F2	tggccaggcctgtcttaaactc	ttcttaggtccgtgggtttttacg	65°C	1.5	-
C4F3	aaagtgctgggattacaggtgtgag	tcgataatcccgagaactc	55°C	1.0	+
C4F4	gaaccataggactttgactgatgg	actgatggttatccgggttcccttg	65°C	1.5	-
C4F5	agctgttgagctgtcatcacaatcc	gaatttcctacccgtctatggtagg	65°C	1.5	-
C5F1	tagtggggagtggggtcttagcttc	tcgttcaccctcctttcttcttacc	58°C	1.5	-
C5F2	gggcatgttgaaatatacccagtc	tctgagtaatagaggggtacgttgg	65°C	1.5	-
C5F3	cttgccagcgtgacagtg	cgaaaccccgtggagtag	65°C	1.5	-
C5F4	caggttggggctcctcgtcatactg	cttccgacgtgacttaaggaaagag	65°C	1.5	-
C5F5	gcttgtctcaactttcactgtc	gaatttcctacccgtctatggtagg	65°C	1.5	-
C6F1	tggtattcaggaggatgcag	acctacgatcgtaaaaagt	65°C	1.5	-
C6F2	cccatcaatgcttatttgttctt	gcctgggagacggagact	65°C	1.5	-
C6F3	tgggctgtttgagttttgttc	cggtgtaaaaggttcgtgac	65°C	1.5	-

*T_a annealing temperature; ^†Mg⁺⁺-concentration is given in mM; ^‡DMSO was added at 5% f.c.

ISSN: 1471-2407