Figure 1

Carbohydrate specificity of GS-I and VVA lectins. Reactivity of GS-I and VVA lectins with an array of carbohydrate probes was tested by a standard ELISA. Carbohydrate probes include: 1, GalNAcα 1→3GalNAcα-PAA; 2, Galβ1→4GlcNAcβ-PAA; 3, Galβ1→3GalNAcα-PAA; 4, GlcNAcβ1→3Galβ-PAA; 5, Galα1→3Galβ-PAA; 6, GlcNAcβ1→4GlcNAcβ-PAA; 7, β-GalNAc-PAA. The starting concentration of each lectin was 10 μg/ml that was further serially diluted. The assay was repeated three times with similar results. SD is shown for reactive sugars only.