Figure 5
From: Knockdown of TFIIS by RNA silencing inhibits cancer cell proliferation and induces apoptosis
Western analysis indicates reduced TFIIS protein after TFIIS siRNA treatment. For the Western analysis, MCF7 cells were treated with vehicle, S-siRNA or TFIIS siRNA#2 as indicated. TFIIS and alpha-tubulin monoclonal antibodies were employed to detect their respective proteins. The ratio of TFIIS to alpha-tubulin as determined by imagequant (BioRad) is depicted by the bar-graph and listed as well above the columns. Cells were collected 48 hours after initial siRNA treatment.