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Table 2 Effect of modulators of IL-6 synthesis in human colon carcinoma cell clones

From: Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells

 

IL-6 secretion by human colon carcinoma cell clones

Addition

Caco-2/AQ

COGA-1A

COGA-13

 

pg/ml

fold change

pg/ml

fold change

pg/ml

fold change

None

4.86 ± 0.49

1.00 ± 0.10

5.49 ± 0.96

1.00 ± 0.17

21.20 ± 1.56

1.00 ± 0.07

indomethacin

 

0.88 ± 0.36

 

0.87 ± 0.25

 

1.02 ± 0.27

PGE2

 

1.03 ± 0.30

 

1.08 ± 0.11

 

1.79 ± 0.20***

1,25-(OH)2D3

 

1.13 ± 0.14

 

1.72 ± 0.17**

 

1.20 ± 0.03*

17β-E2

 

0.98 ± 0.08

 

1.26 ± 0.24

 

0.67 ± 0.12**

IL-1β

 

2.96 ± 0.26***

 

0.85 ± 0.13

 

1035 ± 73***

IL-1β plus indomethacin

 

3.12 ± 0.28***

 

0.93 ± 0.28

 

1147 ± 197***

IL-1β plus PGE2

 

2.83 ± 0.42***

 

0.92 ± 0.29

 

1366 ± 210***

IL-1β plus 17β-E2

 

2.62 ± 0.24***

 

0.79 ± 0.12*

 

1071 ± 33***

  1. Additions to culture medium for 24 h were: indomethacin, 10-6 M; PGE2, 10-7 M; 1,25-(OH)2D3, 10-8 M; 17β-E2, 10-7 M; IL-1β, 5 ng/ml.