Figure 4
Effect of 1,25-(OH)2D3 on rhIL-6-induced inhibition of differentiation of Caco-2/AQ cells. Incubation time was 72 h. Concentrations in culture medium: 1,25-(OH)2D3, 10-8 M; rhIL-6, 100 ng/ml. Cellular differentiation was evaluated from activity of the marker enzyme alkaline phosphatase. Data were calculated as mU/μg cellular protein and expressed as "percent of control" (means ± SD, n = 8). Statistically significant differences: *, P < 0.05; ***, P < 0.001 (Student's t-test).