Figure 4

Correlation between promoter methylation and RFC mRNA levels. The relative level of RFC mRNA in each cell line is shown on the "x" axis as folds relative to that of CCRF-CEM (arbitrarily defined as 1), as measured by quantitative real-time RT-PCR. The extents of RFC promoter B methylation was shown on the "y" axis as percentage relative to that of SssI methyl transferase treated genomic DNA (arbitrarily defined as 100%), as measured by quantitative real-time MSP. The cell lines (n = 8) are indicated above each of the data points, respectively. A reverse correlation (Correlation coefficient = -0.59, p < 0.05) was obtained in this panel of cell lines.