Table 1 Quantitative RT-PCR primer sequences, amplicon length and annealing temperature
Gene | Primer sequences | Amplicon length | Annealing temperature |
|---|---|---|---|
MBD-1 | 5' TCTGGTTGCCAAGGTCCAAA 3' 5' ACATCCATCTTCCCTTCCCGA 3' | 122bp | 61°C |
vimentin | 5' TGGAAGAGAACTTTGCCGTTG 3' 5' AAGGTGACGAGCCATTTCCTC 3' | 101bp | 60°C |
GRP78 | 5' CACCAATGACCAGAATCGCCT 3' 5' CAATGCGCTCCTTGAGCTTT 3' | 101bp | 60°C |