Figure 5
From: Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway
Neutralization of IFN-β partly attenuates poly I:C-improved tumor apoptosis. (A) IFN-β protein level is measured by WB in lysates of Hela cells or MCA38 cells cultured with poly I:C/CHX for the indicated time periods. β-actin is shown as a loading control. (B) Hela cells or MCA38 cells were treated with anti-human IFN-β antibody or anti-mouse IFN-β antibody at the concentration of 1 × 104 U/ml for 4 hours before being stimulated with poly I:C (100 μg/ml)/CHX (1.5 μg/ml) for 12 hours. Apoptosis were determined as above. The data is presented as the mean ± SD.