Table 2 Primers, restriction enzymes and fragment lengths for IVS24-9delT, 5557G>A and IVS38-8T>C variants
From: Association of common ATMvariants with familial breast cancer in a South American population
Variant | Primer secuence (1) | Anealing temperature | Restriction enzyme | Fragment lengths |
|---|---|---|---|---|
IVS24-9delT | F 5' ACTAAGCTGCTGGTCTGAAC 3' R 5' GTCCTGGAACAATCTTAAAGC 3' | 48°C | FnuH I | T allele : 176 bp + 24 bp (-T) allele: 199 bp |
IVS38-8T>C | F 5' ATGGTAATGGCCTAGACTGG 3' R 5' ATCCAAGTTTGCAGGGGTTG 3' | 58°C | Rsa I | T allele: 295 bp + 148 bp C allele: 260 bp + 148 bp + 35 bp |
5557G>A | F 5' TAATATGTCAACGGGGCATG 3' R 5' ATTTCTCCATGATTCATTTGGAT 3' | 52°C | Rsa I | G allele: 156 bp + 23 bp A allele: 179 bp |