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Figure 3 | BMC Cancer

Figure 3

From: Activation of Akt is increased in the dysplasia-carcinoma sequence in Barrett's oesophagus and contributes to increased proliferation and inhibition of apoptosis: a histopathological and functional study

Figure 3

Functional leptin receptor expression by OE33 cells. Expression of the long (Ob-Rb), short (Ob-Ra) and common sequence leptin receptors was detected by RT-PCR using specific primers (3a). Surface expression of Ob-R was detected using immunocytochemistry with an anti-Ob-R antibody, primary antibody binding was recognised using a donkey-FITC conjugated ant-rabbit antibody and fluorescence microscopy (3b top panel). No staining was detected in the absence of anti-Ob-R antibody (3b bottom panel) (× 63 magnification). Leptin increased cell numbers in OE33 cells (3c): serum-starved OE33 cells were treated with increasing concentrations of leptin and relative cell numbers assessed after 24 hours using the MTT colourmetric assay, results expressed as mean ± SEM, N = 3, *P < 0.05 vs basal.

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BMC Cancer

ISSN: 1471-2407

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