Figure 5

Elevated IFN-γ levels after intratumoral injection of nonviral mIL-18 plasmid DNA. Splenocytes from CT26 tumor-bearing mice after gene therapy were collected at 4, 7, and 14 days after gene treatment, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006).