Figure 3
From: Aberrant over-expression of a forkhead family member, FOXO1A, in a brain tumor cell line
a) Summary of FOXO1A cDNA sequencing and analysis. The structures of two FOXO1A splice variants (FOXO1A-2 and FOXO1A-3) are indicated. Two previously identified (*), and three additional single nucleotide differences were identified (see Table 3 for more details). b) Identification of alternative FOXO1A transcripts. RT-PCR analysis of the PER-453 cell line using FOXO1A specific primers (see Fig. 2b) generated amplicons of 1364 bp (canonical FOXO1A), 872 bp (FOXO1A-2), and 838 bp (FOXO1A-3). RT-PCR products were electrophoresed on 0.8% ethidium bromide stained agarose gels.