Figure 3

RT-PCR of CYYR1 mRNA isoforms. An example of agarose gel loaded with duplicated RT-PCR products: B2M (lanes 1–6, 586 bp), CYYR1 CAG^- (lanes 7–12, 321 bp), and CYYR1 CAG⁺ (lanes 13–18, 324 bp) mRNAs for samples NE 16, NE 17 and NE 18, respectively from left to right. DNA M5 marker (M1 and M2, 250 ng and 500 ng, respectively) were used for quantification by Gel Doc 2000 software.