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Figure 2 | BMC Cancer

Figure 2

From: Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats

Figure 2

Effects of a leucine-rich diet on the expression of initiation factors in gastrocnemius skeletal muscle from pregnant, tumour-bearing rats. Western blot images and arbitrary densitometric values for the expression of eIF2α (A) and eIF5 (B). Aliquots of gastrocnemius muscle homogenates were loaded onto SDS-PAGE gels (5 μg protein/well) and, after electrophoresis, the proteins were transferred to nylon membranes and blotted with antibodies to eIF2α and eIF5 (1:750 dilution). For details, see Methods. Abbreviations are defined in the legend to Figure 1. The columns are the means ± S.E.M. of at least 10 rats per group. There was no variation in the expression of actin (42 kDa). * P < 0.05, for comparison WL vs W and PL vs P.

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BMC Cancer

ISSN: 1471-2407

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